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. 2009 Jan;149(1):245–257. doi: 10.1104/pp.108.129353

Figure 3.

Figure 3.

Generation of a tetraploid line segregating for VRN-A2 and VRN-B2. A, Introgression of the nonfunctional vrn-Am2 allele from DV92 into tetraploid wheat. Black bars represent T. monococcum chromosomes, and gray bars represent T. turgidum (T. t.) chromosomes. B, First backcross to tetraploid wheat variety Langdon. C, Marker-assisted selection of line BC1#2 carrying the T. monococcum vrn-Am2 allele. D, Second backcross to tetraploid winter wheat variety Durelle. E, Selection of winter growth habit BC2F2 plants (3:1 segregation of winter to spring). F, Backcross of a selected winter BC2F2 plant carrying the vrn-Am2 allele to T. turgidum subsp. dicoccon accession PI470739 carrying the vrn-B2 deletion. G, Selection of winter BC3F2 plants (recessive vrn-A1 and vrn-B1 alleles). H, Codominant molecular marker for the vrn-B2 deletion from PI470739 based on tightly linked gene SNF-B2. I, Selected line BC3F2-521 heterozygous for VRN-A2 and VRN-B2 loci. J, Graphical representation of chromosome 5A from BC3F2-521. The T. monococcum chromosome 5Am segment carrying the vrn-Am2 allele was recombined with T. turgidum chromosome 5A between the VRN-A1 and VRN-Am2 genes. S, Spring growth habit; W, winter growth habit.