Figure 9.

Subcellular localization of glutenin subunits. Tobacco leaf protoplasts were transiently transformed with a plasmid encoding either the B11-33-HA (A–D) or the C(25, 230)S-HA (E–H) proteins and fixed with 4% paraformaldehyde 48 h after trasfection. The subcellular distribution of the two proteins and of the ER marker BiP was examined by confocal laser scanning microscopy using a combination of an anti-HA monoclonal antibody and a rabbit anti-BiP antiserum. A and E, Distribution pattern of the heterologous proteins revealed by the anti-HA and Alexa Fluor 488 goat anti-mouse antibodies. B and F, Reticular fluorescence of endogenous BiP visualized by the anti-BiP and Alexa Fluor 568 goat anti-rabbit secondary antibody. C and G, Chlorophyll autofluorescence. D, Overlaid images: A (green) + B (magenta). H, Overlaid images: E (green) + F (magenta). n, Nucleus; v, central vacuole. Scale bar = 5 μm.