Table III.
Results of overexpression of Bln1 in cultivars Clansman (compatible) and C.I. 16151 (incompatible) after inoculation with Bgh 5874
| Cultivar (R Gene) | Construct |
Bgh 5874 (AVRa6, avra13)
|
|||
|---|---|---|---|---|---|
| Total No. of GUS Cells with Conidiosporea | Total No. of GUS Cells with Elongating Secondary Hyphaea | PEb | P (Control Versus Bln1)c | ||
| % | |||||
| Clansman (Mla13) | Ubi:GUS | 281 | 84 | 29.9 | |
| Clansman (Mla13) | Ubi:GUS + pUBI:Bln1 | 505 | 273 | 54.1 | 0.0028 |
| C.I. 16151 (Mla6) | Ubi:GUS | 263 | 0 | 0 | |
| C.I. 16151 (Mla6) | Ubi:GUS + pUBI:Bln1 | 372 | 1 | 0.3 | n.s. |
a
Raw numbers indicate the combined results of three independent experiments.
b
PE represents GUS-stained cells with secondary hyphae among the total number of GUS-stained cells with spores attached.
c
P values were obtained using a generalized linear mixed model to test for significant differences in secondary hyphae formation by comparing the test constructs versus the empty vector negative control. n.s., Not significant.