Figure 6.

Complementation of the M. truncatula dmi1-1 mutant (C71) with Os-POLLUX using A. rhizogenes-mediated hairy root transformation (Boisson-Dernier et al., 2001). A full-length cDNA clone of Os-POLLUX (AK067564) was cloned into a binary vector modified from pHellsgate8 driven by the 35S promoter (Helliwell et al., 2002). The binary vector was introduced into the A. rhizogenes strain, ARqua1, and transformed into the roots of the dmi1-1 mutant. A to D, Wild type. E to H, The dmi1-1 mutant transformed with 35S:Os-POLLUX. A and E, Nodule phenotypes of wild-type and transgenic dmi1-1 roots, respectively. B and F, GUS staining of nodules shown in A and E, respectively. C and G, Toluidine blue staining of sectioned nodules from wild-type and transgenic roots, respectively. D and H, Close-ups of the sectioned nodules.