Figure 4.
Reduced expression of rice chr 8 OsGLP gene members correlates with increased rice blast disease in both T0 and T1 plants. Rice blast disease score was assessed in individual T0 and T1 transgenic plants at 7 d after inoculation using a scale from 0 (no mycelia or colonization) to 7 (extensive mycelial growth and colonization). Total chr 8 OsGLP gene expression for each T0 and T1 independent plant was the sum of the relative amounts of mRNA for each constitutively expressed OsGLP (band intensity ratio of transgenic-wild type) and normalized with the band intensity of the internal control EF1-α for each plant.