Table III.

Relative expression profiles of genes involved in mitorespiration or chlororespiration as well as of a component of PSII determined by real-time RT-PCR

Relative expression levels were assessed by the ΔΔC_t method using two biological and technical replicates for each gene. As references for nucleus- and plastome-encoded genes, ubiquitin-conjugated enzyme 21 (At5gG25760; Czechowski et al., 2005) and a ribosomal protein (RPL23.2; AtCg01300) were used. In all cases, sd values of the individual C_t levels were less than 10% of the mean values. The relative transcript levels are expressed as log₂ ratios. In columns 1 and 2, the relative expression levels were referred to the time point 2 h in the light for the wild type (1) or adg1-1/tpt-1 (2); in column 3, expression levels in adg1-1/tpt-1 were compared with the wild type at each time point. Genes up- or down-regulated relative to the respective controls are indicated with roman or italic numbers, respectively. In addition, up- or down-regulated genes above or below a threshold of ±1 are indicated with boldface numbers.

Light/Dark Series	AOX1a			PTOX			NDH1 (NDH-N)			NDH1 (CRR6)			Cytochrome b559 (PsbE)
	1	2	3	1	2	3	1	2	3	1	2	3	1	2	3
2 h of light	–	–	−0.51	–	–	−0.37	–	–	−1.54	–	–	−0.32	–	–	−0.01
8 h of light	−0.55	−0.71	−0.66	−0.41	−0.61	−0.57	−1.43	−0.11	0.21	−1.24	0.77	1.28	0.31	0.39	0.09
12 h of dark	−1.33	−3.35	−2.52	−0.17	−0.50	−0.70	−1.54	−0.08	0.35	−0.32	1.51	1.11	−0.09	0.05	0.08
16 h of dark	−2.84	−3.47	−1.14	0.08	−1.62	−2.07	−2.93	−1.91	−0.09	0.08	0.04	−0.76	−0.18	0.10	0.28