Figure 6.

Polar auxin transport and 1-NPA sensitivity. A, Inhibition of auxin transport in Aucsia-silenced seedlings. In the top row, [³H]IAA was applied between the cotyledons and after 5 h the seedlings were cut in 5-mm segments for radioactivity measurements. In the bottom row, total radiolabeled [³H]IAA was transported to roots of wild-type (WT) and Aucsia-silenced tomato seedlings. The values are sums of the radioactivity measured in the segments located between 3 and 6 cm from the [³H]IAA application point. B, 1-NPA sensitivity of tomato seedlings. The number of adventitious roots per seedling in Aucsia-silenced (L276 1-1 and 7-1) lines and in wild-type plants treated with either 0.05 or 0.1 μm 1-NPA or without 1-NPA is shown. Error bars represent sd. * P < 0.05, ** P < 0.01.