Figure 6.

Effects of AtNSP1 on the hydrolysis of allylglucosinolate in vitro. A, Chemical structures of hydrolysis products formed from allylglucosinolate: 1, simple nitrile; 2, isothiocyanate; 3, epithionitrile. B to D, Hydrolysis products in enzyme assays carried out with purified AtNSP1 (B), purified ESP (C), or myrosinase alone (D) in 50 mm MES buffer, pH 6.0, containing 2 mm allylglucosinolate. Depicted are GC-MS chromatograms (total ion current traces) of dichloromethane extracts. IS, Internal standard; peak numbers refer to the structure numbers in A.