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. Author manuscript; available in PMC: 2009 Jan 5.
Published in final edited form as: Exp Hematol. 2007 Feb;35(2):240–251. doi: 10.1016/j.exphem.2006.09.016

Figure 3. CA microsatellite validation of A-CGH results.

Figure 3

Cryptic chromosomal abnormalities undetectable by traditional cytogenetics and identified by A-CGH were also verified using a quantitative CA microsatellite PCR assay. A. A-CGH identified a gain of RP1-225E12 sequences on chromosome 6q in patient #28 (left panel, black bar). Quantitative microsatellite PCR for CA microsatellite repeat D6S1699 also detected the duplication (right top panel). The signal intensity ratios for both fluorescent channels are shown in detail for this region (right bottom panel). The lines have been traced for ease of viewing. B. #23 was determined to have a loss of RP11-753M10 sequences on chromosome 13q by A-CGH (left panel, bottom right panel). The deletion was verified by quantitative PCR analysis of CA microsatellite D13S1279 copy number (right top panel).