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. Author manuscript; available in PMC: 2009 Dec 31.
Published in final edited form as: J Immunol Methods. 2008 Sep 29;339(2):264–269. doi: 10.1016/j.jim.2008.09.004

Figure 3.

Figure 3

(A) Plaque assay of virus collected after passaging on primary mouse cells. (B) Replication of RV1A RNA in primary human and mouse BE cells. Cells were infected with 10 PFU/cell of HRV 1A and, at different time points, the viral RNA replication was analyzed by real time PCR. (C) Western blot assay with polyclonal antibodies to RV 3C protease to analyze synthesis of non-structural viral proteins in mock- (M) and RV-infected mouse and human BE cells.