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. Author manuscript; available in PMC: 2009 Oct 10.
Published in final edited form as: Life Sci. 2008 Aug 16;83(15-16):557–562. doi: 10.1016/j.lfs.2008.08.003

Figure 5.

Figure 5

GPx activity and cytotoxicity evaluated in J774 macrophage cells. (A) J774 cells were incubated with vehicle, Trolox (1mM) or TIIA (3µM) for 18 hours. Cell lysates were then assayed for total GPx activity. (B) J774 cells were incubated with 3 uM TIIA, or with TIIA plus 3 mM or 4 mM of MS for 18 hours. (C) J774 cells were pretreated for 18 hours with ethanol (vehicle), 3 mM MS, 3 uM TIIA or 3 uM TIIA with 3 mM MS. Cells were then treated with 300 uM H2O2 for 4 hours. Cell death was evaluated by TUNEL stainig, using DPI (Blue) for cellular nuclei and data is presented with respect to untreated cells which were set at 100% viability (not included in this figure). Data is presented as mean ± SEM. For panels A and B, * p< 0.03 versus control; n=3 per group. For panel C, *p<0.02 versus all other conditions; n=3 or 4 per group.