Table 2.

TIIA did not affect caspase activities. J774 cells received no pretreatment (control and H₂O₂ group), 1mM Trolox (Trolox group) and 3µM TIIA (TIIA group) for 18 hours. Then, H₂O₂, Trolox and TIIA groups were incubated with 300µM H₂O₂, whereas the control group was kept in regular medium. After 4 hours, cells were collected and cell lysates were used for measuring caspase activities as described under “Experimental Procedures.” H₂O₂ increased all three caspases activities. Trolox only reduced caspase-8 activity, not caspase-9 or caspase-3. TIIA, however, did not inhibit any of these caspases.

	Pretreatment	H2O2	Caspase-9 (OD/µg protein)	Caspase-3 (pmol/min/µg protein)	Caspase-8 (OD/µg protein)
Control	None	−	2.05±0.39	0.21±0.01	1.73±0.22
H2O2	None	+	7.88±0.04	1.13±0.05	6.30±0.22
Trolox	Trolox	+	7.04±0.89	1.32±0.12	4.92±0.44*
TIIA	TIIA	+	9.37±1.23	1.55±0.26	6.47±0.54

^*p-value < 0.05 versus H₂O₂ group