Fig. 2.

Notch2-deficient kidneys (N2) develop distal tubules without formation of podocytes and proximal tubules. (A, B) Wild type (A) kidney contains high Wilms tumor-1-expressing cells in glomerular podocytes and S-shaped bodies. The only cells that express low level of Wilms tumor-1 in mutant (B) are mesenchymal cells surrounding cytokeratin8-expressing ureteric buds (red). (C, D) No LTL-stained proximal tubules are found in mutant (C), compared to wild type (D). (E, F) Mutant kidney (E) develops numerous E-cadherin-positive, cytokeratin8-negative distal tubules, some of which are connected to cytokeratin8-positive ducts (dash line). The wild type proximal tubules, judged by morphology, also express E-cadherin (arrowhead in F). Wt: wild type. N2: Notch2 mutant. Bar, 0.1mm (A, B, E’) and (C to F).