Fig. 6.

Notch1-deficient ES cells contribute to various parts of the nephron. The kidneys are harvested from E16.5 embryos (A-F) or adult (G), and subject to whole mount β-gal staining. (A, C, E) Wild type ES cells in E16.5 kidneys are found in early nephrons (blank arrowhead in A), proximal convoluted tubules (PCT, circle in C), and glomerular podocytes (blank arrowhead in E). (B, D, F, G) Notch1-/-; Rosa26 ES cells contribute to renal vesicles (arrow in A), S-shaped bodies (blank arrowhead in A) and podocytes in the capillary-loop stage (star in B). (D, D’) Notch1-deficient ES cells (blue) contribute to LTL-labeled PCT together with wild type cells (unstained). One LTL-labeled cross-section that composed entirely from Notch1-deficient cells is shown in D (arrowhead). (E) Glomerular podocytes develop in the absence of Notch1 (blank arrowhead). The circle indicates the capillary lumen with red blood cells in it. (G) The adult kidney is stained with LTL (brown). Some of the LTL-labeled tubules in the inner cortex are entirely derived from Notch1-/- cells (solid arrow). Blank arrowhead and sold arrowhead indicate a juxtamedullary glomerulus and a cortical glomerulus, respectively. Gray arrowhead in C, D insets mark the duct.