Fig. 7.
Notch1-deficient metanephroi are phenotypically wild type. The genotypes are marked above all panels. Where indicated, floxed alleles were recombined with the Pax2-Cretg/+ strain. (A,B,E,F) Whole mount staining of LTL (green) and CK8 (red) detects extensive renal tubulogenesis. Loss of Notch1 did not alter proximal tubule formation (D) whereas loss of RBP-J resulted in loss of LTL-positive epithelial cells. Note that loss of Notch1 or RBP-J in the duct (see Notch1 staining in inset, C-D) did not prevent duct branching. (C,D,G,H) Glomerular podocytes are labeled with WT-1 (green). In (G, H) whole mount preparation is also stained with CK8 (red). WT-1High, synaptopodin-positive (red in C,D, inset in G) Glomeruli are present in wild type (C), Notch1 deficient metanephroi (D), and RBP-J heterozygotes (G) but not in RBP-J deficient metanephroi (inset in H, no WT-1 or synaptopodin) (Bar, 0.5mm).