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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Pediatr Res. 2008 Nov;64(5):477–481. doi: 10.1203/PDR.0b013e3181827c2c

Figure 2.

Figure 2

cAMP and cGMP PDE activities are greater in the mature (■) than in the immature ductus (□) arteriosus. Ductus homogenates were incubated in assay buffer containing either 3H-cAMP (panels A, B, and C) or 3H-cGMP (panels D and E). PDE activity was measured either in the absence (basal) or presence of selective PDE inhibitors (cilostamide 1×10−6 M, for PDE3; rolipram 5×10−6 M, for PDE4; and MY5445 5×10−6 M, for PDE5); or activators (CaCl2/calmodulin (4×10−3 M)/10 units, respectively), for PDE1). We determined the PDE activity for each PDE family (Δ) by subtracting the basal activity from the activity in the presence of inhibitor or activator. Basal1: no EGTA was added to the assay buffer in CaCl2/calmodulin experiments (see Methods). N= number of separate animals used (immature = 5; mature = 5). (*): p<0.05, immature compared with mature.