Table 1.
Distribution of Structures in E. faecium Peptidoglycan
| Species | Theoretical Massa | Measured Mass | Percentage [%]b,c | Percentage [%]c,d |
|---|---|---|---|---|
| Monomers | 47 | 30 | ||
| 1 | 695.2856 | 695.2855 | <1 | <1 |
| 2 | 823.3805 | 823.3802 | 22 | 13 |
| 3 | 894.4177 | 894.4174 | 5 | 3 |
| 4 | 938.4075 | 938.4071 | 13 | 8 |
| 5 | 965.4548 | 965.4555 | <1 | <1 |
| 6 | 1009.4446 | 1009.4446 | 6 | 4 |
| 7 | 1080.4817 | 1080.4825 | <1 | <1 |
| Dimers | 43 | 51 | ||
| 8 | 1814.8151 | 1814.8150 | 8 | 10 |
| 9 | 1885.8522 | 1885.8510 | 3 | 4 |
| 10 | 1929.8421 | 1929.8420 | 23 | 27 |
| 11 | 1956.8894 | 1956.8899 | 2 | 2 |
| 12 | 2000.8792 | 2000.8784 | 7 | 8 |
| 13 | 2071.9163 | 2071.9158 | <1 | <1 |
| Trimers | 8 | 15 | ||
| 14 | 2806.2497 | 2806.2450 | 2 | 3 |
| 15 | 2921.2767 | 2921.2767 | 5 | 10 |
| 16 | 2992.3138 | 2992.3140 | 1 | 2 |
| Teramers | 2 | 4 | ||
a
Theoretical masses were calculated with Xcalibur™ 2.0
b
Percentages with respect to monomers, dimers, trimers, and tetramers
c
Both percentages include structural variations of the species (see Table 2). Under optimized digestion conditions, for data from triplicate runs of four different sample preparations, each species contribution was within +/− 3% of the values listed here.
d
Percentages with respect to total number of peptidoglycan stems