Figure 3. The effect of NT is dependent on [Ca2+]i stimulation and PKC activation.
A. HCT116 cells were pretreated with BAPTA/AM (30 μM) for 20 min, and then treated with of NT (100 nM) for 2 h; RNA was extracted and analyzed by RPA using the hCK-5 multi-probe. B. HCT116 cells were seeded in plates in McCoy’s 5A medium with FBS. Two days later, the media was changed to serum free for 24 h, pretreated with BAPTA/AM (30 μM) for 20 min, and then treated with NT (100 nM) for 6 h. The conditioned media was collected and IL-8 secretion was measured by ELISA. (Results are shown as mean ± SD and representative of three separate experiments; * = p < 0.05 vs control, † = p < 0.05 vs NT only). C and D. HCT116 cells were pretreated with 1 μM of ionomycin, A23187, Gö6983, Ro-31-8220 or GFx for 20 min, and then treated with NT (100 nM) for 2 h. RNA was extracted and analyzed by RPA using the hCK-5 multi-probe.