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. Author manuscript; available in PMC: 2009 Jan 5.
Published in final edited form as: Clin Cancer Res. 2006 Sep 15;12(18):5346–5355. doi: 10.1158/1078-0432.CCR-06-0968

Figure 6. IL-8 and NT increase HCT116 cell migration.

Figure 6

HCT116 cells (5 ×104) were seeded in the upper well of the Costar Transwell System as described in “Materials and Methods”; vehicle, IL-8 (2.5 ng/mL) (A), NT (100 nM) or NT + curcumin (10 μM). (B) were added to the lower well. After 16 h incubation, the cells on the lower surface of the well were fixed in methanol and stained with 1% crystal violet. The average number of migrated cells (n = 3) was quantified by counting under a microscope in at least 7 different viewing fields at 10x magnification. (Results are shown as mean ± SD and representative of separate experiments; * = p < 0.05 vs control).