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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Cell Immunol. 2008 May 16;251(2):93–101. doi: 10.1016/j.cellimm.2008.04.007

Fig. 3. IL-15 snap arming occurs before T cell maturation to a Gzm B+ effector phenotype, happens prior to proliferation, and is perforin-independent.

Fig. 3

(A–B) The cytotoxic protein granzyme B was monitored by flow cytometry in CD3+ T cells after stimulation with IL-15. IL-15 treated and untreated T cells were stained with either anti-Gzm B (A) or an isotype control antibody (B). The interleukin 15 and cytokine-free controls had negligible Gzm B staining profiles that were similar to the isotype control label. (C) Cells were labeled with CFSE and placed in culture with or without IL-15 stimulation. After 1 day, both groups of T cells demonstrated similar CFSE label with few, if any, proliferating cells that would have had lower CFSE staining. (D) Perforin WT and perforin−/− splenocytes were cultured for 1 day with 50 ng/ml IL-15 and then cytotoxicity was redirected against P815 target cells. The WT cells had 3–4 fold more snap-armed cytotoxicity than the perforin−/− cells.