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. Author manuscript; available in PMC: 2009 Jun 4.
Published in final edited form as: Oncogene. 2008 Aug 11;27(53):6720–6728. doi: 10.1038/onc.2008.286

Figure 4. Treatment with TSA markedly decreases the interactions of the IgH enhancers with thebcl-2 promoter region.

Figure 4

(a) The effect of TSA treatment on the interactions of the IgH 3' enhancer BamHI sites 4 through 8 with the bcl-2 promoter region BamHI site 1 in DHL-4 cells was determined by 3C analysis. DHL-4 cells were treated with 500 ng/ml TSA for 18 hours. The PCR signal was normalized to the PCR efficiency derived from a parallel analysis of 3C samples of BAC DNA clones as well as to the association of the BamHI sites at the GAPDH locus in the untreated or treated samples.

(b) The effect of TSA on the interactions of the IgH 3' enhancer BamHI sites with the bcl-2 promoter region BamHI site 2 in DHL-4 cells was determined by 3C analysis as described for (a).