Table 2.
Molecular Diversity Indices | Tajima's D | Fu's F | Mismatch Distribution | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
n | No. | S | k (var) | h ± SD | π ± SD | D | P | F | P | SSD | P (SSD) | τ | θ0 | θ1 | Age in My | |
COI | ||||||||||||||||
clade A | 35 | 8 | 8 | 0.756 (0.325) | 0.556 ± 0.094 | 0.00066 ± 0.00017 | -1.791 | 0.0125 | -3.403 × 1037 | 0.000 | 0.00872 | 0.252 | 0.781 | 0.000 | 99999 | 0.52 |
grade B | 17 | 7 | 9 | 2.132 (1.555) | 0.853 ± 0.053 | 0.00181 ± 0.00032 | -0.717 | 0.264 | -23.81 | 0.000 | 0.0136 | 0.406 | 1.129 | 1.276 | 105.547 | 0.75 |
clade A + grade B | 52 | 14 | 16 | 2.141 (1.463) | 0.782 ± 0.053 | 0.00187 ± 0.00023 | -1.306 | 0.0801 | -26.995 | 0.000 | 0.00585 | 0.761 | 3.457 | 0.000 | 3.905 | 2.30 |
cytB | ||||||||||||||||
clade A | 35 | 9 | 7 | 0.965 (0.453) | 0.692 ± 0.054 | 0.00185 ± 0.00029 | -1.393 | 0.0725 | -30.526 | 0.000 | 0.108 | 0.172 | 1.139 | 0.004 | 99999 | N/A |
grade B | 17 | 6 | 6 | 1.309 (0.737) | 0.779 ± 0.073 | 0.00236 ± 0.00045 | -0.880 | 0.217 | -28.290 | 0.000 | 0.004 | 0.675 | 1.408 | 0.000 | 99999 | N/A |
clade A + grade B | 52 | 14 | 10 | 2.016 (1.330) | 0.830 ± 0.030 | 0.00387 ± 0.00034 | -0.546 | 0.334 | -27.010 | 0.000 | 0.00150 | 0.855 | 2.02 | 0.533 | 9.341 | N/A |
Number of samples (n), number of haplotypes (No.), number of polymorphic nucleotide sites (S), average number of nucleotide differences (k) and variance, haplotype diversity (h) and standard deviation, and nucleotide diversity (π) and standard deviation calculated for each gene in the two largest and most widespread clades of Lampides boeticus. Tajima's D and Fu's F test statistics with probability values for deviation from neutral evolution and test statistics for mismatch distributions of each gene (Fig. 3), with estimates of time since expansion. Reliable estimates for cytB divergence rates are not available for the calculation of time since expansion. Significant P values are bold.