Skip to main content
. Author manuscript; available in PMC: 2009 Jan 5.
Published in final edited form as: Dev Dyn. 2006 Feb;235(2):315–326. doi: 10.1002/dvdy.20626

Figure 2.

Figure 2

RP-HPLC/RIA analysis of whole body extracts during the third larval instar in Drosophila. The ecdysteroid content, as measured by RIA using either the H22 (black) or SHO3 antisera (red) is shown for each HPLC fraction (1 ml) in selected samples as indicated (in hours after ecdysis to the third instar). (VPP), very polar products; (E), ecdysone; (20E), 20-hydroxyecdysone; (26E), 26-hydroxyecdysone; (20,26E), 20,26-dihydroxyecdysone; (3D20E), 3-dehydro-20-hydroxyecdysone; (20E'), 3-α-epi-20-hydroxyecdysone; (MakA), makisterone A; (20dMakA), 20-deoxymakisterone A (24-methylecdysone); 44, combined larval and white puparial (44WP) extracts; (WP + 4 hr), pre-pupae 4 hr after formation of white puparia.