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. Author manuscript; available in PMC: 2009 Jan 5.
Published in final edited form as: Dev Dyn. 2006 Feb;235(2):315–326. doi: 10.1002/dvdy.20626

Figure 3.

Figure 3

Whole body titer of 20-hydroxyecdysone during the third larval instar in Drosophila. The concentration of 20E was determined using RIA with the SHO3 antisera following sample resolution by RP-HPLC. A correction for 20E cross-reactivity was made, i.e. a 5-fold increase relative to E. Sgs3-GFP denotes the time that the Sgs3-GFP fusion protein is first significantly expressed in the larval salivary glands. (W), beginning of wandering; (~50% WP), about 50% of animals had formed white puparia (WP) by 44 hr after ecdysis. Extracts of larvae and pupae at 44 hr were combined for this analysis. (WP + 4), pre-pupae 4 hr after formation of white puparia.