Table 1.
Neutralizing activity of V3 mAbs against a panel of subtype B viruses
| Virusa | IC50(μg/ml)b |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 19b | 39F | C011 | F2A3 | F530 | LA21 | LE311 | B4e8 | 447D | 58.2 | |
| QH0515 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
| QH0692 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | 4.1 | 40.3 | 39.3 |
| BG1168c | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
| SS1196c | >50 | >50 | >50 | >50 | >50 | >50 | >50 | 34.6 | 1.9* | 0.5* |
| 5768-p27 | 3.2 | >50 | >50 | >50 | 5.9 | 32.7 | 41.2 | >50 | >50 | >50 |
| 6101-p15 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
| 91US056 | >50 | >50 | >50 | >50 | 14.8 | >50 | >50 | >50 | >50 | >50 |
| 92BR020c | 21.5 | 1.8 | 1.4 | 2.6 | 2.4 | 1.2 | 2.6 | 0.4 | >50* | >50* |
| 92BR021 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
| 92HT594c | >50 | >50 | >50 | >50 | 43.2 | >50 | >50 | 4.3 | 7.9 | >50 |
| 92US712c | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | 0.6* | 1.4* |
| 93TH305 | >50 | 20.3 | 19.3 | 36.2 | 13.3 | >50 | 13.4 | 37.4 | 8.5 | 25.4 |
| JR-CSFc | >50 | >50 | >50 | >50 | >50 | >50 | >50 | 7.2 | >50 | 32.8 |
| JR-FLc | >50 | >50 | >50 | >50 | >50 | >50 | >50 | 2.4 | 32.6 | >50 |
| ME1 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
|
| ||||||||||
| aMLV | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
a
All viruses are quasispecies pools except where noted. Virus designations are generally denoted as year (of isolation), country (of isolation), and name/number. aMLV, amphotrophic murine leukemia virus (negative control virus). c, molecular clone.
b
IC50s are color-coded as follows: white, IC50 >50 μg/ml; blue, 50 μg/ml> IC50 >10 μg/ml; yellow, 10 μg/ml> IC50 >1 μg/ml; red, IC50 <1 μg/ml. The IC50s for mAbs B4e8, 447-52D (447D), and 58.2 are taken from a previous studies (Binley et al., 2004; Pantophlet et al., 2007).
*
, IC50 obtained with quasispecies pool (mAbs 447D and 58.2 only); for these viruses the IC50 against the molecular clone listed has not been determined.