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Functional shift of TSGP2_R85G and TSGP3_G85R mutants. A) Binding of U44619 by TSGP2_R85G and TSGP3_G85R mutants as measured by microcalorimetry. Protein (2 µM) was titrated using 20 µM ligand. B) Inhibition of collagen-induced platelet aggregation by TSGP2_R85G and TSGP3_G85R mutants.