C5 binding to TSGP2 mutants. A) C5 binding to immobilized T2WT (310 RU final immobilized level), T2M4 (174 RU final immobilized level) and T2ML (251 RU final immobilized level). B) C5 binding to immobilized T2WT (channel 2: 533 final immobilized level) and T2H95D (channel 4: 572 final immobilized level). C) Binding kinetics for C5 to immobilized T2H95D. A single binding site model fit is indicated by the dashed line. Curves from bottom to top, indicate 8, 16, 33, 66, 132, 263, 526 nM, respectively.