Table 1.

Thermodynamic parameters for binding of arachidonic acid, U44619, cTXA₂ and LTB₄ by moubatin, TSGP2 and TSGP3. Also indicated is binding of 5-HT and histamine by “moubatin-like 3”.

Protein	na	ΔHb	TΔSb	Kdc	ΔGb
Arachidonic acid
Moubatin	2.2	−12.0 ± 0.2	−1.6	34 ± 6	−10.4
TSGP2	1.0	−13.0 ± 0.2	−3.0	69 ± 7	−10.0
TSGP3	0.7	−3.5 ± 0.2	6.5	59 ± 24	−10.0
U46619
Moubatin	0.9	−19.0 ± 0.3	−8.3	20 ± 3	−10.7
TSGP2	NBd	NBd	NBd	NBd	NBd
TSGP3	1.2	−13.3 ± 0.1	−2.1	9 ± 1	−11.2
TSGP2_R85G	1.0	−13.0 ± 0.1	−1.7	7 ± 1	−11.3
TSGP3_G85R	NBd	NBd	NBd	NBd	NBd
cTXA2
Moubatin	1.0	−20.9 ± 0.3	−10.3	24 ± 4	−10.6
TSGP2	NBd	NBd	NBd	NBd	NBd
TSGP3	1.0	−18.0 ± 0.1	−6.5	5 ± 0.7	−11.5
LTB4
Moubatin	0.6	−13.5 ± 0.2	−2.8	16 ± 3	−10.7
TSGP2	1.0	−16.7 ± 0.2	−6.0	18 ± 3	−10.7
TSGP3	1.0	−7.7 ± 0.1	2.9	21 ± 5	−10.6
TSGP2_R85G	1.0	−10.7 ± 0.1	0.3	10 ± 1.6	−11.0
TSGP3_G85R	1.0	−9.1 ± 0.3	0.1	228 ± 36	−9.2
T2_ML	0.9	−14.0 ± 0.2	−3.0	12 ± 2	−11.0
T2_M4	1.0	−16.3 ± 0.1	−4.9	6 ± 0.8	−11.4
T2_H95D	0.9	−14.4 ± 0.2	−3.3	12 ± 2	−11.1
Serotonin
Moubatin-like 3	1.0	−18.7 ± 0.3	−9.1	116 ± 13	−9.6
Histamine
Moubatin-like 3	2.1	−15.9 ± 0.1	−6.2	106 ± 3	−9.7

^aValues indicate the stoichiometry of ligand binding.

^bValues are given as kcal/mol. S.E.M. represents the deviation of the experimental data from the fitted data.

^cValues are given as nanomolar.

^dNB No binding detected at 2µM protein and 20µM ligand. S.E.M. represents the deviation of the experimental data from the fitted data.