For all of the untreated samples (lanes 1–10), upon RT-PCR, bands corresponding to mRNA of cIAP1 (354 bp), NAIP (446 bp) and XIAP (360 bp) were detectable, whereas no bands for cIAP2, survivin and p53 were detected. All samples (lanes 1–10), except the negative control samples (lane N), revealed bands of β-actin (350 bp). Similar results were observed for mineral oil-treated pouch tissues. Lane M: molecular weight markers.