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Upon direct sequencing of the PCR product of p53 (370 bp), a point mutation of C to G was noted for the hamster buccal-pouch tissue specimens treated with 7,12-dimethylbenz[a]anthracene (DMBA) for 3 and 7 weeks, as compared with the untreated tissue specimens (arrow). A great variation of the sequence of p53 for hamster buccal-pouch tissue specimens treated with DMBA for 14 weeks as compared with the untreated pouch tissue specimens was found.
