Table 2.
Primers | Sequences | Product (bp) | Accession no. |
---|---|---|---|
cIAP1 | Forward 1: 5-CAG CCT GAG CAG CTT GCA A-3′ | 354 | BC016174 |
Reverse 1: 5-GCC CAT TTC CAA GGC AGA T -3′ | 674 | ||
Forward 2: 5′ TTA TTT GTG GAT AAG AAT ATG AAG 3′ | |||
Reverse 2: 5′ AAA AAT CCT TAT TTT AAA ACA CAA 3′ | |||
cIAP2 | Forward 1: 5-TCC GTC AAG TTC AAG CCA GTT-3′ | 328 | BC037420 |
Reverse 1: 5-TCT TTT TCC TCA GTT GCT CTT TCT CT-3′ | 832 | ||
Forward 2: 5′ AGA AGA TGA AAT AAG GGA AGA GGA G 3′ | |||
Reverse 2: 5′ TTT TAC TTC ACT TAC AAT TTC AAT AAT 3′ | |||
NAIP | Forward 1: 5-GCT TCA CAG CGC ATC GAA-3′ | 446 | U80017 |
Reverse 1: 5-ATG AGA GAC CCA AAA TCC GAA A -3′ | 650 | ||
Forward 2: 5′ AAA AAG AAA AGA AAA GAA AAG AAA AAT 3′ | |||
Reverse 2: 5′ TCT ACT AAT TCT TTT TTT TCT TTT TT 3′ | |||
XIAP | Forward 1: 5-AGT GGT AGT CCT GTT TCA GCA TCA-3′ | 360 | U45880 |
Reverse 1: 5′-GTT CCT CGG GTA TAT GGT GTC TGA-3′ | 884 | ||
Forward 2: 5′ TTT AGG TGA AGG TGA TAA AGT AAA GTG 3′ | |||
Reverse 2: 5′ TTC ACA TCA CAC ATT CAA TCA 3′ | |||
Survivin | Forward 1: 5-AGA ACT GGC CCT TCT TG GA-3′ | 200 | BC016174 |
Reverse 1: 5-AAG GAA AGC GCA ACC GGA CG-3′ | 584 | ||
Forward 2: 5′ AAT AAG AAG AAA GAA TTT GAG GAA A 3′ | |||
Reverse 2: 5′ AAA AAA ACA CAA CAA CAA AAA AAC T 3′ | |||
β-actin | Forward 1: 5-AGA ACT GGC CCT TCT TGG A-3′ | 350 | X-00351 |
Reverse 1: 5-AAG GAA AGC GCA ACC GGA CG-3′ |
cIAP1, cellular inhibitors of apoptosis protein 1; cIAP2, cellular inhibitors of apoptosis protein 2; NAIP, neuronal apoptosis inhibitory protein; XIAP, X-linked inhibitors of apoptosis protein.