Skip to main content
. 2008 Oct;89(5):309–320. doi: 10.1111/j.1365-2613.2008.00583.x

Table 2.

Oligoprimers 1 used for RT-PCR and oligoprimers 2 used for PCR-based methylation assay

Primers Sequences Product (bp) Accession no.
cIAP1 Forward 1: 5-CAG CCT GAG CAG CTT GCA A-3′ 354 BC016174
Reverse 1: 5-GCC CAT TTC CAA GGC AGA T -3′ 674
Forward 2: 5′ TTA TTT GTG GAT AAG AAT ATG AAG 3′
Reverse 2: 5′ AAA AAT CCT TAT TTT AAA ACA CAA 3′
cIAP2 Forward 1: 5-TCC GTC AAG TTC AAG CCA GTT-3′ 328 BC037420
Reverse 1: 5-TCT TTT TCC TCA GTT GCT CTT TCT CT-3′ 832
Forward 2: 5′ AGA AGA TGA AAT AAG GGA AGA GGA G 3′
Reverse 2: 5′ TTT TAC TTC ACT TAC AAT TTC AAT AAT 3′
NAIP Forward 1: 5-GCT TCA CAG CGC ATC GAA-3′ 446 U80017
Reverse 1: 5-ATG AGA GAC CCA AAA TCC GAA A -3′ 650
Forward 2: 5′ AAA AAG AAA AGA AAA GAA AAG AAA AAT 3′
Reverse 2: 5′ TCT ACT AAT TCT TTT TTT TCT TTT TT 3′
XIAP Forward 1: 5-AGT GGT AGT CCT GTT TCA GCA TCA-3′ 360 U45880
Reverse 1: 5′-GTT CCT CGG GTA TAT GGT GTC TGA-3′ 884
Forward 2: 5′ TTT AGG TGA AGG TGA TAA AGT AAA GTG 3′
Reverse 2: 5′ TTC ACA TCA CAC ATT CAA TCA 3′
Survivin Forward 1: 5-AGA ACT GGC CCT TCT TG GA-3′ 200 BC016174
Reverse 1: 5-AAG GAA AGC GCA ACC GGA CG-3′ 584
Forward 2: 5′ AAT AAG AAG AAA GAA TTT GAG GAA A 3′
Reverse 2: 5′ AAA AAA ACA CAA CAA CAA AAA AAC T 3′
β-actin Forward 1: 5-AGA ACT GGC CCT TCT TGG A-3′ 350 X-00351
Reverse 1: 5-AAG GAA AGC GCA ACC GGA CG-3′

cIAP1, cellular inhibitors of apoptosis protein 1; cIAP2, cellular inhibitors of apoptosis protein 2; NAIP, neuronal apoptosis inhibitory protein; XIAP, X-linked inhibitors of apoptosis protein.