Figure 5. Co-activation of serotonin receptors and mGluRs synergistically activates p38 MAP kinase in PFC slices.

A, Western blot analysis of activated (Thr¹⁸⁰/Tyr¹⁸²-phosphorylated) p38 MAPK (top) and total p38 MAPK (bottom) in lysates of PFC neurons treated without (–) or with 10 min of 5-HT (40 μm), glutamate/glycine (Glu, 50 μm/5 μm) or 5-HT + Glu (left). Alternatively, PFC neurons were treated without (–) or with 10 min of 5-HT (40 μm), DHPG (100 μm), or 5-HT + DHPG (right). Note the strong activation of p38 with 5-HT co-applied with Glu or DHPG. B, quantification of p38 activation with different treatments. Each bar represents mean ±s.e.m. of 4–5 independent experiments. *P < 0.005, ANOVA.