Figure 5. Somatic Ca²⁺ does not increase during inspiratory drive.

A, average inspiratory drives from neurons (n = 56) loaded with Fluo-4 (n = 29) or OGN5N (n = 27) aligned to inspiratory drive onset (arrow). B, ΔF/F was computed for each of n traces (as indicated) from a single neuron, ± 20 ms of inspiratory onset, then averaged. Analysis of variance was performed on individual traces. There were no differences in variance before and after inspiratory drive onset in any condition (Student's t test). Ba, Fluo-4, P = 0.94; Bb, OGB5N, P = 0.30; Bc, Fluo-4 + QX-314, P = 0.46.