Figure A1.
Fluorescence intensity of fluo-4 and rhod-2 during a Ca2+ transient Line-averaged intensities of fluorescence in line scan images of fluo-4 or rhod-2, recorded simultaneously on the same voltage-clamped cell, upon depolarization for 30 ms to +20 mV. The photomultiplier gains were adjusted so that the initial average intensity would be the same for both dyes. Plotted are averages of raw intensity, not normalized to resting fluorescence. The crossing of records after the pulse is inconsistent with one-pool models of the dye reactions. See Appendix text for details. Identifier 042408b, record 10.