A, response to NMDA (10 μm) and glycine (10 μm) applied for 2 min, followed by 5 min of SKF-82958 (20 nm) and spiperone (2 nm), and finally NMDA (10 μm), glycine (10 μm), SKF-82958 (20 nm) and spiperone (2 nm) for 2 min. B, mean current (±s.e.m.) of the first NMDA response compared to the second response in the presence of SKF-82958 and spiperone (n= 9, P > 0.05). C, mean percentage SKF-82958 inhibition (±s.e.m.) in experiments with only ATP and GTP in the pipette solution (Fig. 1A) compared to experiments in the presence of 50 μm intracellular dynamin inhibitory peptide (unpaired t test P < 0.05).