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. 2008 Aug 28;586(Pt 20):4825–4842. doi: 10.1113/jphysiol.2008.158709

Figure 4. The presence of β2/3, γ2 and δ subunits in rat adrenal medulla.

Figure 4

A, electrophoresis of PCR products of rat brain and adrenal medulla cDNAs for β1, β2, β3, γ1, γ2S (arrows), γ2L, γ3, and δ subunits and β-actin. PCR products of 341, 317, 355, 360, 374, 398, 255 and 333 bp for β1, β2, β3, γ1, γ2S, γ2L, γ3 and δ subunits, respectively, were observed in brain cDNAs, whereas β2, β3, γ2S and δ PCR products were observed in adrenal medulla cDNAs. PCR products for β-actin are 198 bp. B, immunoblots for β2/3, γ2 and δ subunits of homogenates of rat brain (b) and adrenal medulla (m). 53 kDa β2/3, 52 kDa γ2 and 50 kDa δ proteins were detected in brain and adrenal medulla by mouse anti-β2/3 mAb (BD17), rabbit anti-γ2 Ab and rabbit anti-δ Ab (Chemicon), respectively.