Figure 8. xTRP1 RNAi suppresses SOCE in rods but not cones.

A, TRPC1 immunoblots from control and RNAi-treated retinas. The transfected retina shows a decrease in a ∼70 kDa band corresponding to TRPC1; B, immunostaining of control (Ba) and RNAi-treated retinas with the TRPC1 antibody shows a reduction in the RNAi signal. The staining was confined to narrow stripe within the OPL (arrow in Bb). C, Ca²⁺ imaging of depletion-evoked signals from control (transfecting reagent only), rods incubated with the ‘Silencer’ control RNAi and xTRP1 RNAi- transfected rod ISs at 24 h and 48 h incubation. Cells were loaded with 5 μm fura-2 AM and their stores depleted in 0 Ca²⁺+ thapsigargin. Bar graph amplitudes denote peak Ca²⁺ overshoots following return to control saline. D, Ca²⁺ imaging from ISs of control, ‘Silencer’ control RNAi and xTRP1 RNAi-transfected fura 2-loaded cones at 24 h and 48 h incubation. No significant differences were observed in cones from control, ‘Silencer’-treated or transfected retinas.