Figure 5.
Effect of antisense oligodeoxy nucleotides against c-Myc (A), E2F1 (B), c-Myc and E2F1 (C), and c-Myb (D) on the expression of Akt, mTOR, c-Myb, and COX-2 in Alexander HCC cell line. Cells were subjected to serum deprivation for 24 h, followed by treatment with antisense oligodeoxy nucleotides (AODN) against c-Myc, E2F1, and c-Myb. Experiments were repeated at least 3 times, the results were reproduced in HuH1 and HuH7 HCC cell lines. AODN, antisense oligodeoxy nucleotides; SCR, scramble.