Table 2007.05A.
Interlaboratory study results for aristolochic acid I in botanicals and dietary supplements potentially contaminated with aristolochic acid I by LC-UV with confirmation by LC/MS
| Aristolochic acid I | Avg., μg/g | Sra | RSDrb, % | SRc | RSDRd, % | No. of outlier labs | HorRat | No. of labs used |
|---|---|---|---|---|---|---|---|---|
| Aristolochia manschuriensis, stem | 2610 | 44.9 | 1.72 | 222 | 8.51 | 2 | 1.7 | 8 |
| Clematis armandii, stem | 6.94 | 0.654 | 9.42 | 0.850 | 12.3 | 1 | 1.0 | 9 |
| Tablets 20 μg/g high fortification with Aristolochia spp., root | 12.8 | 2.08 | 16.3 | 2.53 | 19.8 | 0 | 1.8 | 10 |
| Aristolochia spp., root | 1290 | 23.8 | 1.85 | 69.7 | 5.42 | 0 | 1.0 | 10 |
| Clematis armandii, stem, 10 μg/g low fortification with Aristolochia spp., root | 206 | 0.754 | 7.31 | 0.875 | 8.48 | 0 | 0.8 | 10 |
| Stephania tetrandra, root, 10 μg/g low fortification with Aristolochia spp., root | 10.0 | 1.18 | 11.8 | 1.82 | 18.2 | 0 | 1.6 | 10 |
| Akebia trifoliata, stem, 10 μg/g low fortification with Aristolochia spp., root | 7.83 | 0.518 | 6.61 | 0.807 | 10.3 | 0 | 0.9 | 10 |
| Tablets 10 μg/g low fortification with Aristolochia spp., root | 6.58 | 0.875 | 13.3 | 0.875 | 13.3 | 2 | 1.1 | 8 |
| Stephania tetrandra, root, 20 gμ/g high fortification with Aristolochia spp., root | 16.7 | 0.844 | 5.04 | 1.16 | 6.92 | 3 | 0.7 | 7 |
| Akebia trifoliata, stem, 20 μg/g high fortification with Aristolochia spp., root | 15.0 | 0.790 | 5.25 | 2.05 | 13.7 | 0 | 1.3 | 10 |
| Clematis armandii, stem, 20 μg/g high fortification with Aristolochia spp., root | 20.5 | 0.849 | 4.14 | 1.68 | 8.18 | 0 | 0.8 | 10 |
| Akebia trifoliata, stem | <2.00 | NAe | NA | NA | NA | 1 | NA | 9 |
| Stephania tetrandra, root | <2.00 | NA | NA | NA | NA | 0 | NA | 10 |
a
Sr = Standard deviation for repeatability.
b
RSDr = Relative standard deviation for repeatability.
c
SR = Standard deviation for reproducibility.
d
RSDR = Relative standard deviation for reproducibility.
e
NA = Not applicable.