Table 2007.05E.

Chromatographic conditions for LC/MS ion trap

HPLC column	Zorbax SB-C18, 3 mm id × 150 mm, Agilent
Mobile phase A	0.1% (v/v) formic acid and 0.1% (w/v) ammonium acetate in DI water
Mobile phase B	0.1% (v/v) formic acid and 0.1% (w/v) ammonium acetate in methanol
Pump program
Time, min	%A	%B	Gradient curve
0	80	20	NAa
20	0	100	1b
21	80	20	1
25	80	20	NA
Column temperature, °C	40
Flow rate, mL/min	0.5
Injection volume, μL	30
Sample introduction	Ion trap
Mode
Mass range scan	m/z 80 to 370
Discharge current, μA	5
Heated capillary temperature, °C	150
Vaporization temperature, °C	450
Sheath gas setting	70
Aux gas setting	20
Ions to be monitored for LC/MS ion trap
Compound	Parent ion (m/z)	Ions	Isolation width (m/z)	Relative collision energy, %
Aristolochic acid I	359	298	2	30
Aristolochic acid I	298.0 (from product ion above)	251, 252, 268	2	35

^aNA = Not applicable.

^bA gradient curve of 1 indicates a linear transition.