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PAGE analysis of purified rPPK. After electrophoresis on an SDS/4–15% polyacrylamide gradient gel, proteins were visualized by Coomassie blue staining. Purification steps of rPPK as described in Materials and Methods: lane 1, lysate pellet (25 μg); lane 2, solubilized membrane fraction (5 μg); lane 3, nickel-affinity-purified rPPK (5 μg). Arrow indicates position of rPPK.
