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Enzymatic activities of wild-type and mutant rPPKs
| Protein | PolyP synthesis, units × 10−6/mg | ATP synthesis, units × 10−6/mg | Autophosphorylation, mol of Pi/mol of PPK monomer |
|---|---|---|---|
| rPPK | 9.2 | 1.0 | 0.19 |
| H430Q | 9.7 | 1.1 | 0.15 |
| H441Q | <0.01 | 0.007 | <0.0001 |
| H460Q | <0.01 | 0.005 | <0.0001 |
| H598Q | 8.3 | 1.3 | 0.10 |
| H441A | <0.01 | 0.03 | <0.0001 |
| H460A | <0.05 | 0.03 | <0.0001 |
