Figure 3. Multiple alignments of RAMP groups.

RAMP proteins were clustered in two different groups according their vertebrate class origin being mammals or fishes. A—Thirty-two mammal RAMP sequences were aligned by T-Coffee software (Notredame et al. 1998). The sequences from Orictolagus cuniculus and Loxodonta africana were excluded because these partial sequences perturbed analyses. B—Twenty-six fish RAMP sequences were aligned as above except the RAMP protein from Tetraodon nigroviridis, which was a partial sequence and also disturbed the analyses. Amino acid numeration and secondary structure are based on previous RAMP analyses (Benitez-Paez, 2006).