Skip to main content
. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Nat Immunol. 2008 Aug 24;9(10):1171–1178. doi: 10.1038/ni.1646

Figure 4. Naip5 is required for activation of caspase-1 and release of IL-1β induced by L. pneumophila.

Figure 4

(a) Cell death assayed by release of lactate dehydrogenase (LDH) from peritoneal macrophages infected by wild-type (WT) or flagellin-deficient (ΔflaA) L. pneumophila (MOI = 1). Chr13-AJ mice carry chromosome 13 and the Naip5 locus from A/J mice on the B6 background. (b) Immunoblot of processed p10 of active caspase-1 detected in supernatants of bone-marrow-derived macrophages infected with wild-type (WT) or flagellin-deficient (ΔflaA) L. pneumophila at an MOI of 1. (c) Sandwich ELISA assay for release of IL-1β ±s.d. from Pam3CSK4-primed wild-type (B6), Naip5-deficient (Naip5-KO) or Naip5-heterozygous (Naip5-het) bone-marrow-derived macrophages infected with wild-type (WT) or flagellin-deficient (ΔflaA) L. pneumophila at an MOI of 1. Results are representative of 2–5 independent experiments.