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. Author manuscript; available in PMC: 2009 Jan 30.
Published in final edited form as: JAMA. 2008 Jan 30;299(4):425–436. doi: 10.1001/jama.299.4.425

Table 3.

Expression of MicroRNAs in Colon Adenocarcinoma Tumors and Colon Adenomas

microRNA Average Difference
in Threshold Cyclea 		SD (Difference
in Threshold Cycle)		 Fold Changeb P
Valuec
MicroRNA Expression in Tumors vs Paired Nontumorous Tissue
From the Hong Kong Validation Cohortd
miR-20a
 1.18
 0.97
 2.3
 <.001
miR-21
 1.47
 1.20
 2.8
 <.001
miR-106a
 1.25
 0.94
 2.4
 <.001
miR-181b
 0.47
 1.03
 1.4
 <.001
miR-203
 0.83
 1.40
 1.8
 <.001
MicroRNA Expression in Adenoma vs Paired Nonadenoma Tissuee
miR-20a
 -0.11
 0.97
 0.9
 .82
miR-21
 0.64
 0.90
 1.6
 .006
miR-106a
 0.28
 1.22
 1.2
 .19
miR-181b
 0.30
 1.24
 1.2
 .27
miR-203 0.77 1.98 1.7 .14
a

Threshold cycle is the unit of measurement in quantitative reverse transcription polymerase chain reaction (RT-PCR) to measure relative gene expression. Average (tumor change in threshold cycle minus paired nontumor change in threshold cycle) or average (adenoma change in threshold cycle minus paired nonadenoma change in threshold cycle) from quantitative RT-PCR. Positive values indicate higher expression in tumor tissue.

b

Calcluated by 2average difference in threshold cycles.

c

Wilcoxon matched pairs test.

d

For the tumor/nontumor comparisons, 113 pairs of tissues were used for miR-20a and miR-203 while 111 pairs of tissue were used for miR-21, miR-106a, and miR-181b.

e

For all adenoma/nonadenoma comparisons, 18 pairs of tissue were used.