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. Author manuscript; available in PMC: 2009 Jan 6.
Published in final edited form as: Cancer Lett. 2006 Jan 18;242(2):207–214. doi: 10.1016/j.canlet.2005.11.007

Figure 2. TGF-β increases Smad2 protein phosphorylation.

Figure 2

(A) Phosphorylation of Smad2 occurred in a time-dependent manner after treatment with TGF-β (1 ng/ml) demonstrating that TGF-β/Smad signaling is intact in SK-N-SH cells. (B) LY294002 treatment resulted in increased phosphorylation of Smad2 by Western blot analysis. This increase was attenuated by GRP treatment. β-actin was used as a protein loading control.