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Neuronal death and DNA fragmentation in acute EAE with time. A: Immunofluorescence staining of neuron-specific cell death on days 8–11 post–EAE induction using the antibodies to NeuN (red), a neuron marker, and TUNEL staining (green), which identified cell death with anti-DIG antibody. Arrows indicate TUNEL-positive neurons (400× magnification). B: Agarose gel electrophoresis of genomic DNA for detection of DNA fragmentation (C, control; E, EAE; D7–D11, days 7–11).
