Calpain and calpastatin levels during EAE. Time-dependent changes in the expression of 80-kDa (A) and 76-kDa (B) calpain, 145-kDa SBDP (C), and 110-kDa calpastatin (D) during acute EAE. Upper panels (A–D) show representative Western blot, and lower panels show the corresponding bar graphs (n ≥ 3 animals per group). β-Actin was used as a control for equal protein loading. Data are presented as percent increase compared with the control set at 100% (C, control; E, EAE; D7–D11, days 7–11).