Involvement of endoplasmic reticulum and caspase-3 activity in apoptosis during EAE. Time-dependent changes in the 55- and 40-kDa caspase-12 (A), 120-kDa SBDP (B), 20-kDa caspase-3 (C), and 85-kDa PARP-1 (D) expression during acute EAE. Upper panels (A–D) show representative Western blots and lower panels show the corresponding bar graphs (n ≥ 3 animals per group). β-Actin was used as a control for equal protein loading. Data are presented as percent increase compared with the control set at 100% (C, control; E, EAE; D7–D11, days 7–11).