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. Author manuscript; available in PMC: 2009 Jan 6.
Published in final edited form as: Mucosal Immunol. 2008 Mar 5;1(3):229–238. doi: 10.1038/mi.2008.1

Figure 6.

Figure 6

Gonococci and purified LOS engage membrane-expressed TREM-2A and induce transmembrane signaling. BWZ reporter cell constructs expressing either TREM-2A (TR-2A) or TREM-3 (TR-3) were used to measure LacZ activity as an indicator of signal transduction following incubation of cells with gonococci or LOS. For stimulation with gonococci, reporter cells were incubated with a bacteria to cell ratio of either 100:1 or 1:1. For LOS, TREM-2A-expressing reporter cells were treated with LOS concentrations of either 100 μg/ml or 1 μg/ml. The LOS and all strains of gonococci induced LacZ activity but to variable degrees. Significantly greater signaling was induced in cells expressing TREM-2A than in those expressing TREM-3. Statistical comparisons: p < 0.05 for all comparisons of lacZ activity induced by gonococci and LOS in TREM-2A-expressing cells versus nontransfected cells; p < 0.001 for the comparisons of lacZ activity induced by 1291 and F62 in TREM-2A-expressing cells versus TREM-3-expressing cells. The bars represent the mean of triplicate data points and the error bars are ±SD. The results are representative of four independent experiments.